Differential Regulation of Endothelial Exocytosis of P-selectin and von Willebrand Factor by Protease-Activated Receptors and cAMP Running Title: Differential PAR and cAMP-dependent exocytosis
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چکیده
*Corresponding author: Department of Pharmacology Vanderbilt University Medical Center 442 Robinson Research Building 23rd Ave. South @ Pierce Nashville, Tennessee 37232-6600 Office:(615) 343-3533 Fax: (615) 343-1084 Email: [email protected] Word Count: 4,681 Scientific Heading: Thrombosis and Vascular Biology Blood First Edition Paper, prepublished online December 6, 2005; DOI 10.1182/blood-2004-07-2698
منابع مشابه
Differential regulation of endothelial exocytosis of P-selectin and von Willebrand factor by protease-activated receptors and cAMP.
Thrombin-mediated endothelial-cell release of von Willebrand factor (VWF) and P-selectin functionally links protease-activated receptors (PARs) to thrombosis and inflammation. VWF release can be stimulated by both Ca2+ and cAMP, and, although both VWF and P-selectin are found in Weibel-Palade bodies (WPBs), we found that their release could be differentially regulated. In these studies, human u...
متن کاملHEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Differential regulation of endothelial exocytosis of P-selectin and von Willebrand factor by protease-activated receptors and cAMP
Thrombin-mediated endothelial-cell release of von Willebrand factor (VWF) and P-selectin functionally links proteaseactivated receptors (PARs) to thrombosis and inflammation. VWF release can be stimulated by both Ca2 and cAMP, and, although both VWF and P-selectin are found in Weibel-Palade bodies (WPBs), we found that their release could be differentially regulated. In these studies, human umb...
متن کاملDifferential actions of PAR2 and PAR1 in stimulating human endothelial cell exocytosis and permeability: the role of Rho-GTPases.
Endothelial cell proteinase activated receptors (PARs) belong to a family of heterotrimeric G protein-coupled receptors that are implicated in leukocyte accumulation and potentiation of reperfusion injury. We characterized the effect and the signal transduction pathways recruited after stimulation of endothelial PAR2. We used von Willebrand Factor (vWF) release and monolayer permeability to per...
متن کاملAutocrine regulation of endothelial exocytosis: von Willebrand factor release is induced by prostacyclin in cultured endothelial cells.
Vascular endothelial cells respond to external stimuli by altering the secretion of several bioactive molecules, including von Willebrand factor (vWf), prostacyclin (PGI2) and nitric oxide (NO). The release of all three molecules is regulated by a rise in cytosolic calcium ([Ca2+]i). In the present study we investigated whether cAMP-dependent signaling provides differential regulation of these ...
متن کاملSmall GTP-binding protein Ral is involved in cAMP-mediated release of von Willebrand factor from endothelial cells.
OBJECTIVE von Willebrand factor (vWF) is synthesized by endothelial cells and stored in specialized vesicles called Weibel-Palade bodies (WPBs). Recently, we have shown that the small GTP-binding protein Ral is involved in thrombin-induced exocytosis of WPBs. In addition to Ca2+-elevating secretagogues such as histamine and thrombin, release of WPB is also observed after administration of cAMP-...
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تاریخ انتشار 2005